Development of Novel and Standardized Cytotoxicity Tests for Additively Manufactured Biomedical Devices

Enis, Veronika

Student Work

Development of Novel and Standardized Cytotoxicity Tests for Additively Manufactured Biomedical Devices

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Additive manufacturing (AM) in biomedical engineering is becoming more popular as it offers rapid fabrication for patient-specific implants and devices. Though the use of AM for these applications is increasing, standardized cytotoxicity testing procedures for complex device shapes is not keeping up. We planned a series of cellular adhesion tests and a cytotoxicity test and determined the feasibility of these methods with the use of a polycarbonate printed knee implant sample. The drop test promotes cellular interaction with the sample by placing a drop of cell suspension onto the surface, and the motion test is a direct contact test where the sample is stored in motion in complete medium containing cells. The filter diffusion test is an indirect contact test where the cells and samples are separated by a filter and stored in motion to measure cytotoxicity. Circumstances prevented us from completing replicates of the tests, but initial feasibility tests provided insight into future improvements that can be made to refine and validate our approach.

This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

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