Upon arsenite stress, cells form stress granules (SGs) through the phosphorylation of eIF2alpha through the HRI kinase in order to conserve cellular resources and restrict mRNA translation. It is unclear if other metals cause SGs. Using fluorescent microscopy, we measured SGs in U20S-DS cells and HAP1 cell exposed to acute doses of arsenic, cadmium, and lead. We find that cadmium (Cd) causes stress granule formation likely via activation of the HRI kinase pathway, whereas lead (Pb) does not cause SGs.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Edoh, Enyonam Y.

Subject

• Water
• Sustainability
• Health
• Well-Being
• Innovation

Publisher

• Worcester Polytechnic Institute

Identifier

• 23291
• E-project-050621-163829

Advisor

• Farny, Natalie
• Argüello, José M.

Year

• 2021

UN Sustainable Development Goals

• 15 - Life On Land

Date created

• 2021-05-06

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright

Last modified

• 2022-05-16