The goal of this project was to clone and express the antimicrobial peptide protegrin 1 (PG-1). Initially a yeast system was chosen but was discarded due to technical difficulties. Invitrogen’s bacterial T7 expression system was chosen next to express the peptide. PG-1 expression was verified by anti-his immunoblot and then the peptide was purified by IMAC. Its activity was verified using a Bacillus subtillis radial diffusion assay.

Creator

• Borrelli, Alexander P

Contributors

• Adams, David S.
• Rulfs, Jill
• Bagshaw, Joseph C.

Degree

• MS

Unit

• Biology & Biotechnology

Publisher

• Worcester Polytechnic Institute

Language

• English

Identifier

• etd-0428103-102059

Keyword

• Antimicrobial peptides
• synthetic genes
• protegrins

Advisor

• Bagshaw, Joseph C.

Committee

• Rulfs, Jill
• Adams, David S.

Defense date

• 2003-04-17

Year

• 2003

Date created

• 2003-04-28

Resource type

• Thesis

Rights statement

• In Copyright

Last modified

• 2020-11-27