This project focuses on developing imaging and sensing techniques to detect and characterize low concentrations of Salmonella enterica, with applications in medical and food safety sectors. Initially, bacteria were visualized by staining with the fluorescent dyes propidium iodide and Syto 9. Subsequently S. enterica was genetically engineered to express enhanced green fluorescent protein (eGFP). The expression of eGFP enabled the characterization of bacterial adherence to surfaces using fluorescence microscopy and direct imaging without staining. Surface-Enhanced Raman Spectroscopy was used to characterize bacterial adherence and the organisms were detected from the presence of their unique Raman absorption bands. These SERS surfaces were imaged using Scanning Electron Microscopy which clearly showed the morphology of the electroplated silver. This research aims to advance our ability to detect and study S. enterica biofilms especially for the purpose of improving healthcare and food safety.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Barry, Meghan

Subject

• Industry
• Innovation
• Health
• Food

Publisher

• Worcester Polytechnic Institute

Identifier

• 121297
• E-project-042224-130407

Keyword

• Salmonella
• Green fluorescent protein
• SERS
• Raman spectroscopy
• Biofilms

Advisor

• Lambert, Christopher R.

Year

• 2024

UN Sustainable Development Goals

• 3 - Good Health and Well-being
• 6 - Clean Water and Sanitation
• 9 - Industry, Innovation and Infrastructure

Date created

• 2024-04-22

Resource type

• Major Qualifying Project

Major

• Biochemistry

Source

• E-project-042224-130407

Rights statement

• In Copyright