Chimeric antigen receptor (CAR) T cells are typically engineered using lentiviral insertion of a CAR construct. To overcome potential oncogenesis resulting from random integration in virally engineered constructs, here, we used Cas9 nucleases along with phagemid-derived circular single-stranded DNA to introduce a red fluorescence marker (mCherry) into the TRAC locus and AAVS1 safe harbor locus as a surrogate for a CAR construct, so that the optimal parameters can be identified for prospective use in engineering CAR T cells.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Clift, Maxwell
• Ungashick, Ellie

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-081524-155414
• 124215

Keyword

• Cellular engineering
• Phagemids
• CRISPR
• CAR T cells

Advisor

• Rulfs, Jill
• Ambady, Sakthikumar

Year

• 2024

Date created

• 2024-08-15

Resource type

• Major Qualifying Project

Major

• Biomedical Engineering
• Biology & Biotechnology

Source

• E-project-081524-155414

Rights statement

• In Copyright

Last modified

• 2024-09-19