Student Work
Designing T-Cell Receptor Constructs Using Golden Gate Cloning
PublicDownloadable Content
open in viewerLatent TB infections are a result of the diseases' ability to evade host immune defenses and remain in a quiescent asymptomatic state. Clonal expansion of T-cells within granulomas of individuals with active and latent TB provides an opportunity to determine the antigens capable of promoting an adaptive immune response within a human subject. Using sequence information from T-cells of patients with Latent Tuberculosis, T cell receptor (TCR) variable region inserts were designed. The objective of this work is to utilize Golden Gate cloning of the compiled TCR sequences to create a repertoire of specific TCRs for eventual expression using retroviral vectors.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-042519-065548
- Advisor
- Year
- 2019
- Sponsor
- Date created
- 2019-04-25
- Resource type
- Major
- Rights statement
Relations
- In Collection:
Items
Items
Thumbnail | Title | Visibility | Embargo Release Date | Actions |
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Maxwell_Studley_BBT_MQP_eCDR_Submission.pdf | Public | Download |
Permanent link to this page: https://digital.wpi.edu/show/c821gn13p