Student Work
Cysteine scanning mutagenesis in the Na, K-ATPase
PublicThe catalytic cycle of Na, K-ATPase enzyme has been described in great detail, but the events occurring at the molecular level to perform the energy-coupled ion transport are unknown. To approach this problem we performed site-directed mutagenesis of transmembrane helixes H5, H6 and the loop connecting them. A few residues were individually replaced by cysteine. In order to separate heterologously expressed protein from the endogenous proteins, Histidine-tag was introduced to some of the cysteine mutants. Resulting mutants were then stable expressed in COS cells.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- 01D164M
- Advisor
- Year
- 2001
- Date created
- 2001-01-01
- Resource type
- Major
- Rights statement
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