Effects of Bli-3 on Survival Rates of C. elegans in the Presence of Yeast PathogensPublic
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In C. elegans, the gene bli-3 codes for Duox1, a protein in the NADPH oxidase (Nox) family responsible for cuticle cross-linking. In order to determine if Duox is important in C. elegans host defense, both available bli-3 mutants and C. elegans subjected to RNAi knockdown of bli-3 were exposed to yeast pathogens, and survival assays were performed. One of the bli-3 mutants and the whole body bli-3 RNAi knockout worms showed a significant decrease in survival when exposed to Candida. This indicates that bli-3 does play an immune role in C. albicans infections. Tissue-specific RNAi knockdown was performed in order to determine where this immune function is localized, and knockdowns had similar effects regardless of the tissue.
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