Cell Therapy Process for Volumetric Muscle Loss Public
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Muscle injury therapy employs human primary satellite skeletal muscle cells seeded onto micro-threads. However, the cell culture process prior to seeding that would assure maintenance of the myogenic potential has not been optimized. This cell line has both adherent and non-adherent cell populations; non-adherent population displaying higher myogenic potential. The concentrations of selected components of the cell culture medium were varied to determine their effect on the proportion of non-adherent satellite cells. The subsequent effect on myogenic potential was determined by inducing the cells to differentiate by culture in differentiation medium and calculating the myogenic index. An economic analysis was performed to determine the cost-benefit of implementing the optimized process.
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