Designing T-Cell Receptor Constructs Using Golden Gate Cloning Public
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Latent TB infections are a result of the diseases' ability to evade host immune defenses and remain in a quiescent asymptomatic state. Clonal expansion of T-cells within granulomas of individuals with active and latent TB provides an opportunity to determine the antigens capable of promoting an adaptive immune response within a human subject. Using sequence information from T-cells of patients with Latent Tuberculosis, T cell receptor (TCR) variable region inserts were designed. The objective of this work is to utilize Golden Gate cloning of the compiled TCR sequences to create a repertoire of specific TCRs for eventual expression using retroviral vectors.
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