Etd
Synthetic Genes for Antimicrobial Peptides
公开The goal of this project was to clone and express the antimicrobial peptide protegrin 1 (PG-1). Initially a yeast system was chosen but was discarded due to technical difficulties. Invitrogen’s bacterial T7 expression system was chosen next to express the peptide. PG-1 expression was verified by anti-his immunoblot and then the peptide was purified by IMAC. Its activity was verified using a Bacillus subtillis radial diffusion assay.
- Creator
- 贡献者
- Degree
- Unit
- Publisher
- Language
- English
- Identifier
- etd-0428103-102059
- 关键词
- Advisor
- Committee
- Defense date
- Year
- 2003
- Date created
- 2003-04-28
- Resource type
- Rights statement
- 最新修改
- 2020-11-27
关系
- 属于 Collection:
项目
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