Student Work

SPECIES SPECIFICITY OF NEISSERIA GONORRHOEAE BINDING TO FACTOR H AT COMPLEMENT CONTROL PROTEIN DOMAIN 18-20

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Neisseria gonorrhoeae infects humans in part because of the binding to its surface protein porin (Por) molecules by the complement control protein (CCP) domains 6 and 18-20 of the human complement regulator, factor H (fH). FH activity is thereby localized to the surface of N. gonorrhoeae and results in inactivation of the alternative pathway of complement. This project investigated the Por/fH interaction by creating species-specific sequence changes in human and chimpanzee CCP 20s in cloned fH proteins, and used flow cytometry to determine the ability of the recombinant proteins to bind N. gonorrhoeae. The data indicated that arginine located at position 1203 in fH CCP 20, R1203, is critical for fH-binding to N. gonorrhoeae.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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  • E-project-011009-232512
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  • 2009
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Date created
  • 2009-01-10
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