Recent literature has shown that Matrix and Scaffold Attachment Regions (MARs or SARs) are DNA domains that enhance transcriptional frequency and increase gene expression levels in cells. Research has shown that cell lines containing MARs or SARs are more stable and more productive. Researchers at Abbott Bioresearch Center in Worcester, MA have found two possible MAR regions in an antibody producing cell line. In order to determine whether these regions function as MARs, and if the observed high levels of expression are specific to this cell line, the sequences in question were inserted upstream of the reporter gene, Enhanced Green Fluorescent Protein (EGFP) and tested using FACS analysis. The data indicated the sequences under investigation indeed increased expression of EGFP in stable cell lines, indicating that these sequences can be used to improve transfection and establish high expressing, stable CHO cell lines.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Kedves, Alexia T

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-031405-161010

Advisor

• Adams, David S.

Year

• 2005

Sponsor

• Abbott BASF

Date created

• 2005-03-14

Location

• Worcester

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright