Ethylenediaminetetraacetic acid (EDTA) is widely used in phlebotomy as an anticoagulant drug. There is a need for a simple, reliable, and cheap method to determine the concentration of EDTA in blood collection devices. This project is based on competition kinetics of fluorescence dyes (Rhodamine B, Ru(bpy3)2+, and Fluo-4FF), whose fluorescence intensities are altered [in] the presence of metal ions. In the presence of EDTA the metal ion is chelated and the change in the fluorescence is reversed. The sensitivity of the method is dependent on the relative binding constants of the metal ion with EDTA, the second order kinetics of fluorescence quenching by the metal ion and, in the case of Fluo-4FF the binding constant of the dye for calcium ions.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Jones, Katherine

Publisher

• Worcester Polytechnic Institute

Identifier

• 107981
• E-project-050223-163359

Keyword

• EDTA
• Kinetics
• Fluorescence

Advisor

• Lambert, Christopher R.

Year

• 2023

Date created

• 2023-05-02

Resource type

• Major Qualifying Project

Major

• Chemistry

Source

• E-project-050223-163359

Rights statement

• In Copyright

Last modified

• 2023-06-16