Student Work
Production of biologically active CDK inhibitors p21 and p27 as TAT-fusion proteins
PúblicoCyclin-dependent kinases (CDKs) are proteins that facilitate entrance of a cell through its cell cycle. Proteins p21 and p27 are CDK-inhibitors that arrest the cell cycle at the G0-G1 phase, and induce cell migration. The purpose of this MQP was to construct an expression system for biologically active p21 and p27. A TAT-mediated protein expression method was utilized for expression, and a HepG2 migration assay was developed to assay biological function. The data showed that recombinant proteins TAT-21 and TAT-27 were indeed biologically active in the migration assay.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- 01D248M
- Advisor
- Year
- 2001
- Sponsor
- Date created
- 2001-01-01
- Resource type
- Major
- Rights statement
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