Nanoparticle Delivery of siRNA: Multi-functional Upconverting NaYF4 Nanoparticle siRNA Delivery System for Knockdown of SOD1, a Gene Linked to ALS
公开Upconverting nanoparticles (UCNP) were characterized as siRNA transfection agents, in an approach to target superoxide dismutase 1 (SOD1), a protein implicated in amyotrophic lateral sclerosis. Two UCNP designs were tested: (1) NaYF4:Yb,Er@NaYF4 core/shell nanoparticles with polyethylene imine (PEI) coating and (2) NaYF4:Yb,Er@CaF2 core/shell nanoparticles with a citric acid/PEI coating. The NaYF4-shell particles exhibited a low toxic range, but effective plasmid and siRNA delivery, quantified through green fluorescent protein (GFP) expression and SOD1 knockdown in Western blots. The CaF2–shell particles had a greater working range, but were less effective for payload delivery. Continued research is needed to optimize a UCNP with high cell tolerance and high transfection efficiency.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- 贡献者
- Publisher
- Identifier
- E-project-030912-210054
- Advisor
- Year
- 2012
- Sponsor
- Date created
- 2012-03-09
- Resource type
- Major
- Rights statement
- 最新修改
- 2021-02-02
关系
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项目
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