In-vivo Like Studies of the h-IAPP Amyloid Precursors Using Dielectric Relaxation Spectroscopy

Hirai, Yusuke

Student Work

In-vivo Like Studies of the h-IAPP Amyloid Precursors Using Dielectric Relaxation Spectroscopy

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Recent studies show the amyloid formation in Type II diabetic disease involves aggregation of monomers of the human islet amyloid polypeptide (hIAPP) into oligomers, protofibrils, and fibrils. We present data show Dielectric Relaxation Spectroscopy is a sensitive technique to detect the hIAPP precursors. We measured the dielectric response of the hIAPP and the rIAPP as a function of frequency, temperature, and incubation time. In-vivo like conditions were mimiced by BSA. Our results show the dielectric signal of the hIAPP shifts towards the dielectric signal of the background while the rIAPP did not. The dielectric signal of the hIAPP and the rIAPP shows two relaxation processes over the measured range. We used two Havrilik-Negami functions and conductivity to fit the relaxation processes.

This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

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