Most of the processes in the biomanufacturing industry utilize suspension bioreactor for the production of biologics, whereas there has been a slower development in large scale manufacturing involving adherent cell cultures. This project aims to design a novel bioreactor system which supports adherent cell culture, while improving upon cellular adherence and volumetric efficiency. The final design incorporated a packed bed of microcarriers within a continuous pumping system, thereby promoting bead-to-bead migration and the recycling of unattached cells. The system was tested for proper continuous fluid flow, and the microcarriers were optimized to maximize surface area and cellular attachment potential.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Heng, Anthony
• Kirejczyk, Michael Jon
• Shea, Madison Lee
• Saqib, Solaimon N.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-051820-021224

Advisor

• Sabuncu, Ahmet Can
• Page, Raymond L.

Year

• 2020

Date created

• 2020-05-18

Resource type

• Major Qualifying Project

Major

• Interdisciplinary
• Biomedical Engineering

Rights statement

• In Copyright