This investigation evaluated the effects of protein oxidation on Bacillus subtilis spores that were treated with Fenton reagents. The Fenton reaction produces hydroxyl radicals, which are harmful oxidative agents to biomolecules. The ultimate goal of this Major Qualifying Project (MQP) was to determine if the Fenton Reaction could oxidize spore coat proteins in Bacillus subtilis and if these oxidized proteins could be detected and even quantified by using SDS-PAGE, Western blotting and a spectrophotometric assay.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Palma, Melinda Janet

Publisher

• Worcester Polytechnic Institute

Identifier

• 04D178M

Advisor

• Crusberg, Theodore C.

Year

• 2004

Date created

• 2004-01-01

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright