The process of producing monoclonal antibodies using the hybridoma technology has been an arduous task for many years. In this study, we aim to improve the efficiency of monoclonal antibody production by isolating mature splenic B cells in advance of fusion using magnetic activated cell sorting (MACS) to select for cells expressing CD138, a cell marker present in mature B cells. Pre-isolating a population of cells expressing CD138+, but lacking CD19 and B220, resulted in a reduced number of cells required for fusion.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Vinayak, Jyotsna

Contributori

• Barlow, Eve

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-011110-141433

Advisor

• Adams, David S.

Year

• 2010

Sponsor

• Abbott Bioresearch Center

Date created

• 2010-01-11

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

Ultima modifica

• 2023-09-20