This project investigates the probiotic potential of novel Saccharomyces cerevisiae KTP. S.c. KTP and S.c. 288C growth curves were generated under varying media conditions, including pH, NaCl concentration, and media type. Expression levels were compared using plasmids with different promoter, terminator, and fluorescent protein combinations. KTP exhibited a higher resistance to low pH than 288C but a lower resistance to high NaCl concentrations. Expression levels varied significantly between strains, suggesting more research is needed to characterize KTP’s unique genetic qualities.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• White, Charlotte R.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-050521-232539
• 22586

Advisor

• Young, Eric Mosher

Year

• 2021

Date created

• 2021-05-05

Resource type

• Major Qualifying Project

Major

• Chemical Engineering

Rights statement

• In Copyright

Last modified

• 2021-08-29