Student Work

Microscopy of Yeast and Bacteria

Public

Downloadable Content

open in viewer

Interactions between bacteria and fungi are a driver of many ecosystem functions. Bacteria have been shown to benefit from “fungal highways” as routes through the soil, though many aspects of these interactions are not well understood. Previous work has identified an interaction between the bacterial strain Pseudomonas putida and fungi Lyophyllum atratum. Here, we developed two methods to image both species individually and interacting. On glass slides, fungal hyphae were found to grow and were visualized with non-motile bacteria. On the oat flake agar surface, fungal hyphae did not grow individually, however, this system is promising for maintaining bacterial viability during imaging. This project builds the foundation for future studies to directly visualize bacteria-fungi interactions.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
Creator
Subject
Publisher
Identifier
  • 64706
  • E-project-042722-202646
Advisor
Year
  • 2022
UN Sustainable Development Goals
Date created
  • 2022-04-27
Resource type
Major
Rights statement
License

Relations

In Collection:

Items

Items

Permanent link to this page: https://digital.wpi.edu/show/x633f437s