The purpose of this MQP was two-fold. First, to identify TGF- signaling pathways in PC3 cells and involvement of specific proteins such as PKC. Second, to discriminate between growth inhibitory and extracellular matrix inducing signaling pathways. This was accomplished using three experimental approaches: 1) Cell proliferation assays, 2) Signal transduction/gene expression assays, and 3) Western blot analysis identifying specific isoforms of down-regulated PKC. Although results of the first two approaches were inconclusive, cPKC was down regulated in response to PMA treatment.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Villano, Caren Melissa
• Chen, Michael C.

Publisher

• Worcester Polytechnic Institute

Identifier

• 99D117M

Advisor

• Adams, David S.

Year

• 1999

Sponsor

• University of Massachusetts Medical School, Worcester, MA

Date created

• 1999-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright