The vast amount of bacteria and difficulty in their culturability has impeded progress in classifying and identifying these many unknown species. The purpose of this project was to develop a micro-array blot system to determine the presence and eventually the relative amounts of bacteria in virtually any environment. A hybridization protocol was developed here using florescent (6-FAM) tagged, oligonucleotide probes with fully extracted samples of ribosomal RNA from Proteobacteria of the alpha, beta and gamma sub-classes.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Vitello, Peter Vincent

Publisher

• Worcester Polytechnic Institute

Identifier

• 02D232M

Advisor

• Crusberg, Theodore C.

Year

• 2002

Date created

• 2002-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright