The purpose of this project was to determine whether Ajulemic Acid (AjA), a synthetic analog of tetrahydrocannibinol and gamma-linolenic acid (GLA), an essential fatty acid, induces apoptosis (programmed cell death) in Jurkat cell lines and isolated human lymphocytes. Several methods were used to assay apoptosis: cell morphology, cell viability, cellular DNA fragmentation ELISA, DNA laddering, and caspase-3 enzyme activity. The results indicate that AjA, but not GLA induces apoptosis.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Brown, Lisa Ann

Publisher

• Worcester Polytechnic Institute

Identifier

• 00D134M

Advisor

• Adams, David S.

Year

• 2000

Sponsor

• UMass Medical School

Date created

• 2000-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright