Investigating the Cleavage Specificity of Mycobacterium smegmatis RNase E in vitro
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open in viewerTuberculosis is a bacterial disease caused by Mycobacterium tuberculosis that primarily affects the lungs. The success of M. tuberculosis as a pathogen relies on tight regulation of gene expression related to growth, metabolism, and survival. One strategy the bacterium employs to achieve this is decreasing the rate of mRNA degradation, a process believed to be regulated by RNase E, the major catalytic and scaffolding element of the degradosome. Though thoroughly studied in E. coli, RNase E in M. tuberculosis remains relatively unexplored. In this research, we investigated the cleavage specificity of RNase E using three engineered variants of the enzyme, and collected evidence to suggest that cleavage sites mapped in vivo for the M. smegmatis rne 5’ UTR may be attributed to RNase E activity
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-051820-004059
- Advisor
- Year
- 2020
- Date created
- 2020-05-18
- Resource type
- Major
- Rights statement
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Miniatura | Título | Visibilidad | Embargo Release Date | Acciones |
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Investigating_the_Cleavage_Specificity_of_Mycobacterium_smegmatis_RNase_E_in_vitro_.pdf | Público | Descargar |
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