Paclitaxel (PTX) is one of the leading chemotherapy drugs available for various solid tumor cancers and is most commonly administered systemically in a solution of dehydrated alcohol and Cremophor EL. This project investigated the potential for a Taxus suspension culture derived local delivery system of PTX. For this purpose, increased cell-associated PTX accumulation, DNA removal, and release kinetics were investigated. PTX was retained throughout a piloted decellularization process at an average of 0.68 mg/L with greater than 80% DNA removal. The decellularized biomass allowed for almost complete drug release within 72 hours of suspension. Initial release rate could be sustained when encapsulated in calcium alginate microbeads, suggesting this to be a feasible approach for PTX delivery.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Aldarondo, Dasia Ali
• Robert Dione, Donald Parry
• Werner, Adele

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042519-012948

Advisor

• Coburn, Jeannine M
• Roberts, Susan Celia

Year

• 2019

Date created

• 2019-04-25

Resource type

• Major Qualifying Project

Major

• Chemical Engineering

Rights statement

• In Copyright

Dernière modification

• 2020-11-20