It is difficult to predict the toxic effects of pesticides on humans because traditional 2D cell culture does not provide physiologically relevant data. As a result, we designed a process utilizing 3D cell culture as a model to study the cytotoxicity caused by pesticide exposure. Mn9D cells are well-established as a model of studying neurodegeneration and have not been published in 3D cell culture. Thus, we developed a new approach to form 3D spheroids, with Mn9D cells to model the cellular interactions of neurons. We tested various cell suspension platforms and spheroid characteristics to determine the optimal spheroid. We found that PDMS-coated 96 well plates with 5,000 cells per well formed spheroids with the highest degree of compactness, with an average sphericity of .958 ± .030.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Ippolito, Amanda Nicole
• Digioia, Andrea Michelle
• Atwater, Britney Lee
• DiFelice, Lauren Ashley

Colaboradores

• Dominko, Tanja

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-011414-220402

Advisor

• Rolle, Marsha Whitney

Year

• 2013

Sponsor

• University of Nova Gorica, Slovenia

Date created

• 2013-01-14

Ubicación

• Nova Gorica

Resource type

• Major Qualifying Project

Major

• Biomedical Engineering

Rights statement

• In Copyright

Última modificación

• 2021-02-02