Phosphatidylinositol (PI) has pivotal roles in many cellular signaling events. Due to their anionic nature, these lipids interact with bivalent cations and the cationic parts of proteins. We are investigating the phase behavior of anionic lipids in the presence of Ca2+ or Mg2+ cations using differential scanning calorimetry (DSC) of multilamellar vesicles. For diplamitoylphosphatidyl-inositol (DPPI) vesicles, we found in pH 7.2 buffer a phase transition temperature of 43.6℃, while in the presence of Ca2+ and Mg2+ the phase transition temperature shifted to 55.8°C and 51.6℃, respectively. For Liver phosphatidylinositol (Liver PI), we found in pH 7.2 buffer in the presence of Ca2+ and Mg2+ the phase transition temperature to be 11.8°C and below 0℃, respectively.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Ali, Safiya

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042723-103556
• 105901

Keyword

• Phase Transition Temperature
• Differential Scanning Calorimetry
• Phosphatidylinositol
• Bivalent Cations

Advisor

• Gericke, Arne

Year

• 2023

Date created

• 2023-04-27

Resource type

• Major Qualifying Project

Major

• Management

Source

• E-project-042723-103556

Rights statement

• In Copyright

Last modified

• 2023-06-18