Characterizing IQGAP1-C Domain Binding to Phosphoinositides
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open in viewerIQGAP1 is a ubiquitously expressed protein that is a member of the IQGAP family of scaffolding proteins. IQGAP1 has been found to regulate a diverse range of cellular processes including: cell migration, cell proliferation, cytoskeletal dynamics, and intracellular signaling. Previous studies indicated that IQGAP1 binds to phosphoinositides. Interactions between IQGAP1 and phosphoinositides can be characterized by in-vitro studies using purified proteins and phosphoinositide containing model membranes. Because IQGAP1 is a large multi-domain protein, purifying it presented challenges. Therefore, IQGAP1-C, a truncated protein that includes the proposed PIP binding site, was instead purified. This study shows that we were able to purify IQGAP1-C and characterize its interaction with lipids.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-042418-215625
- Advisor
- Year
- 2018
- Date created
- 2018-04-24
- Resource type
- Major
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Thumbnail | Title | Visibility | Embargo Release Date | Actions |
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CHARACTERIZING_IQGAP1-C_DOMAIN_BINDING_TO_PHOSPHOINOSITIDES.pdf | Public | Download |
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