Bone tissue engineering has many potential applications including healing bone after trauma, repairing and bone defects associated with cancer. However tissue scaffolds are required to create 3D cultures in vitro. Current scaffold fabrication techniques do not allow for adequate control over the internal pore network. With the advent of rapid prototyping fully customized pore structures are feasible. Here we describe the process for creating a biomorphic bone tissue engineering Poly-lactide scaffold using a low cost 3D extrusion printer. We have developed a scaffold that is built fully using a 3d printer and successfully seeded with mouse osteoblast cells using a collagen hydrogel. We have determined that this scaffold has biomimetic geometry and preserves cellular bio-functionality.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Butler, Thomas Sung
• Mayor, Elizabeth Laura
• Halter, Jesse Daniel
• Trabucco, Nicholas Patrick

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042513-041416

Advisor

• Ambady, Sakthikumar
• Granquist-Fraser, Domhnull

Year

• 2013

Date created

• 2013-04-25

Resource type

• Major Qualifying Project

Major

• Biomedical Engineering

Rights statement

• In Copyright