Many genes are known to produce more than one form of mRNA by alternative splicing, in which exons are excluded from an mRNA transcript of a gene, thus altering the conformation of the protein. The dig-1 gene of the nematode Caenorhabditis elegans is involved in neuronal process guidance, and may undergo temporally mediated alternative spicing. Using RT-PCR analysis, two possible sites of alternative splicing were identified. Several RT-PCR products were cloned and sequenced to reveal the actual spicing patterns.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Johansen, Peter Norman

Publisher

• Worcester Polytechnic Institute

Identifier

• 03D120M

Advisor

• Ryder, Elizabeth F.

Year

• 2003

Date created

• 2003-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright