This project, completed at TISVM, is a comparison of activated CF1 mouse oocytes, non-enucleated and chemically enucleated, to discern if chemical enucleation affects cytoplasmic factors, cyclin D and CDK4, or the centrosomal factors,-tubulin and pericentrin. Harvested oocytes were divided into two groups, both activated and the experimental group chemically enucleated. The groups were then compared at specific time points. Immunohistochemistry showed that chemical enucleation has no effect on distribution or concentration of cytoplasmic factors, while removing centrosomal factors.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Caron, Jessica Marie
• Lovett, Rebecca Eileen

Publisher

• Worcester Polytechnic Institute

Identifier

• 02D272M

Advisor

• Rulfs, Jill

Year

• 2002

Date created

• 2002-01-01

Resource type

• Major Qualifying Project

Major

• Biochemistry
• Biology & Biotechnology

Rights statement

• In Copyright