RNA interference (RNAi) is a powerful mechanism for gene silencing that holds great promise for therapeutic applications. To design a drug, scientists must find siRNA sequences capable of strong mRNA silencing and effective methods to deliver the siRNA to target tissues. In situ hybridization techniques enable the visualization of the effectiveness of siRNAs. This report demonstrates the visualization of RNAi mediated gene silencing by using a new, highly sensitive bDNA probe-based in situ hybridization assay known as ViewRNA. The ViewRNA System showed in both a qualitative and semi-quantitative manner the reduction of SOD1 mRNA after cells were transfected with siRNA targeting the gene.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Stanney, William Joseph

Contributors

• Kamens, Joanne

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-050109-131945

Advisor

• Adams, David S.

Year

• 2009

Sponsor

• RXi Pharmaceuticals

Date created

• 2009-05-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

Last modified

• 2023-09-20