Student Work
Expression, purification, and characterization of the M3M4 intracellular region of the human Zn2+ transporter protein, ZIP4
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open in viewerThe goal of this project was to express, purify, and characterize the M3M4 intracellular region of the ZIP4 protein. This region has a series of histidine residues, which have a high zinc binding affinity. The zinc binding affinity and induced conformational change of the M3M4 region was measured using isothermal titration calorimetry and circular dichroism. The result of this project was the standardization and optimization of the protein purification protocol and zinc binding assays, as well as confirmation of previous data.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-042312-193238
- Advisor
- Year
- 2012
- Date created
- 2012-04-23
- Resource type
- Major
- Rights statement
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