Candida infections are common opportunistic infections among humans, especially those with an underlying immunodeficiency. Five species of Candida are most often seen clinically and these include Candida tropicalis, Candida krusei, Candida glabrata, Candida dubliniensis, and Candida albicans. There is growing concern about the emergence of antimicrobial resistant and often misdiagnosed species of Candida albicans and Candida dubliniensis. This study will lay the foundation to study the mycobiome and differentiate between the species of Candida. Clinical isolates were inoculated on CHROMagar for phenotypic pre-identification after 48 h of incubation at 37 °C and genotyped by PCR using species-specific primers ITS regions followed by a PCR-RFLP digestion.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Bartlett, Heather M

Contributors

• Delorey, Toni

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042816-090350

Advisor

• Rao, Reeta Prusty

Year

• 2016

Date created

• 2016-04-28

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright