The scope of this report is to document the methodology used in creating nonsense codons in plasmid DNA by site directed mutagenesis as well as the application in site-specific incorporation of non-natural amino acids into target peptides. A technique to use artificial suppressor tRNA in an in vitro expression system based on a Micrococcus luteus S30 extract is suggested as an alternative to stop codon suppression. Site-specific protein mutagenesis has wide reaching implementations in biochemistry.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Laptewicz, Michael Walter.

Publisher

• Worcester Polytechnic Institute

Identifier

• 01D012M

Advisor

• Bagshaw, Joseph C.

Year

• 2001

Sponsor

• Eikos, LLC

Date created

• 2001-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright