Axonal extension through the site of a peripheral neural degenerative injury is severely limited by the formation of a mature scar. Presently, there are few clinically relevant and cost-effective methods for testing regenerative therapies in mature peripheral neuronal scars. This project aims to design a proof-of-concept cellular connective tissue scar model to evaluate the efficacy of neuroregenerative therapies. To accomplish this, a small-diameter 3D collagen cylinder seeded with NIH-3T3 fibroblasts was prototyped to favor collagen fiber alignment. The tissue was supplemented with ascorbic acid to induce collagen production, simulating an in-vivo scar environment.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Salazar, Fernando
• Richardson, Hanne Marie
• Barbery, Daniela

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042717-143046

Advisor

• Dominko, Tanja
• Page, Raymond L.

Year

• 2017

Date created

• 2017-04-27

Resource type

• Major Qualifying Project

Major

• Biomedical Engineering

Rights statement

• In Copyright