Conditional Loss-of-Growth 1 (CLoG1) is a Novel Protein that Localizes to Microtubules in Physcomitrella patens
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open in viewerUnderstanding the genetic basis of plant growth is important for agricultural improvements. Here, the intracellular localization of CLoG1, a novel protein identified in a temperature sensitive loss-of-growth genetic screen in Physcomitrella patens is investigated. An RNAi complementation assay was used to demonstrate that mEGFP tagged CLoG1 remains functional. Following this, stable lines expressing CLoG1:mEGFP fusions were generated. Imaging by confocal microscopy revealed that CLoG1 associates with the microtubule cytoskeleton, including the mitotic spindle and phragmoplast. A nuclear counting assay was conducted to investigate the participation of CLoG1 in cell division. Though there was no evidence of multi-nucleated cells, the mutation causes a decrease in the number of cells.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-042816-150222
- Advisor
- Year
- 2016
- Date created
- 2016-04-28
- Resource type
- Major
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