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Conditional Loss-of-Growth 1 (CLoG1) is a Novel Protein that Localizes to Microtubules in Physcomitrella patens Public

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Understanding the genetic basis of plant growth is important for agricultural improvements. Here, the intracellular localization of CLoG1, a novel protein identified in a temperature sensitive loss-of-growth genetic screen in Physcomitrella patens is investigated. An RNAi complementation assay was used to demonstrate that mEGFP tagged CLoG1 remains functional. Following this, stable lines expressing CLoG1:mEGFP fusions were generated. Imaging by confocal microscopy revealed that CLoG1 associates with the microtubule cytoskeleton, including the mitotic spindle and phragmoplast. A nuclear counting assay was conducted to investigate the participation of CLoG1 in cell division. Though there was no evidence of multi-nucleated cells, the mutation causes a decrease in the number of cells.

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  • E-project-042816-150222
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  • 2016
Date created
  • 2016-04-28
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