Improving Large Scale Culture of Anchorage Dependent Cells

VanderVliet, Katrina May

Student Work

Improving Large Scale Culture of Anchorage Dependent Cells Public

Chronic diseases are a prevalent and expensive health conditions in the US, with 133 million people suffering from a chronic disease. Tissue engineering, particularly mesenchymal stem cell(MSC) approaches, show great potential in meeting the need for treatments. There is a need for an improved scaffold to promote proliferation of adherent cells within bioreactors. This project aims to solve this gap in technology by constructing a 3-D scaffold of a novel material that would solve production issues of MSC’s. A literature review found materials that were nontoxic, flexible, and allowed cellular adhesion. Experiments quantified cell seeding and proliferation on varying materials to select a material for final design. A multilayer scaffold of stainless steel mesh was selected for final design.

This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

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