Lack of a viable vascular network impedes 3D bioprinting from creating functional tissues. Utilizing the native vasculature of decellularized spinach leaves as a scaffold for cell seeding provides the potential to form a viable vascular network. This network coupled with a gel matrix has the potential to provide the support and nutrients necessary for printing functioning tissues. Our data shows cell viability within a seeded scaffold after 36 hours and demonstrates cellular escape from the spinach leaf scaffold. When combined, the data shows that cells have the ability to adhere and escape from the scaffold, into a gel matrix, mimicking the initial steps for vascular growth.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Lozada, Danilo
• Pomerleau, Alexa
• Mora, Emily

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042518-171058

Advisor

• Gaudette, Glenn R.

Year

• 2018

Sponsor

• DEKA Research and Development Corporation

Date created

• 2018-04-25

Resource type

• Major Qualifying Project

Major

• Biomedical Engineering

Rights statement

• In Copyright