This project investigated differences in the metabolism of CHO cells that had been modified to overexpress a dipeptide transporter (SLC15a1) and IgG1. SLC15a1 and control cultures were grown in media with either glutamine (Q) or alanine-glutamine (AQ) added over 10 days in shake-flasks. VCD and cell culture metabolic data were measured over the duration of the run. Results indicated that SLC15a1 cells grown in Q media maintained a higher VCD compared to the control, while results from the AQ SLC15a1 & control groups were indistinguishable. These observations suggest that SLC15a1 may be regulated by the concentration of monomer peptides such as Q present in media. The results may also suggest that SLC15a1 may be capable of weakly transporting glutamine as well as dipeptides.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Connor, Alexandra Gabrielle

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-121118-005154

Advisor

• Young, Eric Mosher

Year

• 2018

Date created

• 2018-12-11

Resource type

• Major Qualifying Project

Major

• Chemical Engineering

Rights statement

• In Copyright