Muscular dystrophies are a set of very serious muscular diseases that lack treatment as there are no animal models that accurately mimic the human 3D in vivo environment to provide accurate testing in clinical trials of various drugs and therapies. Therefore, our team designed and manufactured a customized cell culture lid for a 96-well plate that interfaces with a programmable Arduino stimulation system to promote muscle maturation. To test the electrical stimulation, bioartificial muscle (BAMs) were developed and stimulated under various regimens to measure the effect on tissue maturation and viability. Based on haemotoxylin and eosin histology stains, stimulated tissue constructs qualitatively showed improved myotube alignment and limited necrosis, compared to unstimulated tissue.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Cunha, Anny Evelen
• Swei, Anisa
• Howard, Ato Agyenkwah
• Jones, Serissa Joelle

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042716-110420

Advisor

• Page, Raymond L.

Year

• 2016

Date created

• 2016-04-27

Resource type

• Major Qualifying Project

Major

• Biomedical Engineering

Rights statement

• In Copyright

License

• All rights reserved