Following discoveries about the role that human basic fibroblast growth factor (bFGF or FGF-2) plays in cell reprogramming events, this project endeavored to establish a mammalian cell platform for further study of these proteins and their functions. FGF2 is encoded by a single mRNA , which utilizes the discrete 5' start sites resulting in production of five FGF2 protein isoforms. The five isoform sequences were originally cloned into separate pBluescript vectors. The sequences were then subcloned into a mammalian expression vector, and the protein was overexpressed in CHO-K1 cells where production and localization were verified by immunoblotting. We report production of two of the five protein isoforms as recombinant human proteins.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Sterling, Alexander Jonathan
• Sternal, Jack Philip
• Clinton, Ryan W
• Richmond, Gregory John

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042611-191936

Advisor

• Dominko, Tanja
• Buckholt, Michael Allan

Year

• 2011

Date created

• 2011-04-26

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright