Adult enhancer factor-1 (Aef-1) is a 4 finger DNA-binding zinc finger protein which functions as an adult alcohol dehydrogenase gene (Adh) repressor in Drosophila. During larvae development it also plays a role in decreasing expression of the Adh gene. This project used mutagenesis of the Aef-1 gene and the bacterial one-hybrid system to examine DNA binding specificity and recognition of the Aef-1 zinc fingers. The intent of this project was to investigate the importance of each zinc finger, and binding specificity of a 3 finger Aef-1 construct.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Braxton, Maceo

Contributors

• Wolfe, Scot

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042607-123741

Advisor

• Adams, David S.

Year

• 2007

Sponsor

• UMass Medical Center

Date created

• 2007-04-26

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

Last modified

• 2023-11-06