INVESTIGATION OF THE DNA-BINDING PROPERTIES OF DROSOPHILA ADULT ENHANCER FACTOR-1 ZINC FINGER PROTEIN Public
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Adult enhancer factor-1 (Aef-1) is a 4 finger DNA-binding zinc finger protein which functions as an adult alcohol dehydrogenase gene (Adh) repressor in Drosophila. During larvae development it also plays a role in decreasing expression of the Adh gene. This project used mutagenesis of the Aef-1 gene and the bacterial one-hybrid system to examine DNA binding specificity and recognition of the Aef-1 zinc fingers. The intent of this project was to investigate the importance of each zinc finger, and binding specificity of a 3 finger Aef-1 construct.
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