Identification of flagellar motility mutants can directly impact cilia-related disease research as flagellar proteins are highly conserved between the green alga Chlamydomonas reinhardtii and humans. Forward and reverse genetic analysis approaches would be more efficient with an available mutant collection and identified sites of gene disruption. This project showed how inserting a DNA fragment into the C. reinhardtii genome resulted in cells with defective swimming. 35 mutants were generated, two of which had insertions in genes of interest. A flagellar protein ODA1 mutant showed decreased protein production, while a calcium ATPase mutant exposed to different calcium concentrations did not show any significant changes in swimming ability.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Rili, Danica Danielle

Contributori

• Brown, Jason
• Witman, George

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042811-124459

Advisor

• Rao, Reeta Prusty

Year

• 2011

Sponsor

• University of Massachusetts Medical School

Date created

• 2011-04-28

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

Ultima modifica

• 2021-02-02