MicroRNAs (miRNAs) are non-coding double stranded RNA molecules known to regulate gene expression. The recent discovery that the miR-30 family of miRNAs overexpress in mouse breast cancer tumor cells in the same regions commonly seen in human breast cancer tumor cells, has lead us to design a system for over-expressing these miRNAs to help evaluate their role in tumorigenesis. In this study, genes encoding miR-30c1, c2, a, and d, were successfully cloned. The gene for miR-30c1 was further investigated in a tet inducible expression system. Fluorescence data indicated HEK-293 cells were successfully transfected with expression plasmid, and qRT-PCR on HEK-293 RNA indicated miR-30c1 levels increased.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Swahn, Samantha K.

Contributors

• Simin, Karl

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-050309-140135

Advisor

• Adams, David S.

Year

• 2009

Sponsor

• University of Massachusetts Medical Center

Date created

• 2009-05-03

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright