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Developing a Device to Investigate Migration of Axon Through a Fibrotic Scar Public

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Peripheral neural degenerative injuries lead to the formation of fibrotic scars at the site of the lesion which limits axonal regeneration resulting in loss of motor and sensory functionality. This project aims to investigate axonal extension through these fibrotic scars. In order to accomplish this, a device of PDMS and nitrocellulose membrane was designed to simulate the site of injury with a fibrotic scar. Fibroblast NIH 3T3 cells were seeded onto the nitrocellulose membrane to simulate the fibrotic scar. This scar was treated Fibroblast Growth Factor 2 (FGF2) in order to create a less dense extracellular matrix composition. To avoid unwanted fibroblast interaction with the neuron culture, the scar was decellularized before implementation into the device.

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Identifier
  • E-project-012816-124947
Advisor
Year
  • 2016
Sponsor
Date created
  • 2016-01-28
Location
  • Nova Gorica
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Permanent link to this page: https://digital.wpi.edu/show/mw22v712f