Of great interest to the pharmaceutical market, GPCR behavior is still not well understood. The interplay between ligand binding, receptor activation, and the resulting cellular response is not well categorized. Current assays to measure activation are indirect and rely on downstream activation of reporter genes. Successful in other GPCRs, a FRET-based direct indicator of activation was developed in Ste2p. Identification of a successful clone will allow further studies of GPCR behaviors, such as fractional occupancy. Pharmaceutical dosing of GPCR-targeted medicines relies on understanding the dynamic relationship of binding, activation, and output that impacts receptor behavior.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Johnson, Victoria Kathleen

Contributori

• Connelly, Sara
• Dumont, Mark

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042618-090854

Advisor

• Heilman, Destin

Year

• 2018

Sponsor

• University of Rochester

Date created

• 2018-04-26

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright