Effects of Point Mutations on the Human Parainfluenza Virus Type-3 Hemagglutinin-Neuraminidase ProteinPublic
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Human Parainfluenza Virus Type-3 is a ssRNA virus belonging to the paramyxovirus family. Paramyxoviruses gain entry into cells through the interaction of the hemagglutinin-neuraminidase (HN) protein and fusion protein. The HN protein performs three functions -- attachment to the host cell, cleavage the fusion protein, and activation of the fusion protein. By creating a variety of point mutations in the HN protein, we observed the interaction of the HN protein with the fusion protein. The hemadsorption assay revealed that all mutations analyzed in this project displayed wild type hemagglutinin activity, however one mutant lost the ability to trigger fusion. This loss of fusion function was not due to a reduced mutant HN protein on the surface since FACs analysis was normal.
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